Development and greenness assessment of a stability-indicating HPLC-DAD method for simultaneous determination of allopurinol and benzbromarone

The growing interest in Green Analytical Chemistry (GAC) principles through the replacement of polluting analytical procedures with greener ones, has encouraged us to develop an eco-friendly stability-indicating HPLC diode array detection method (HPLC-DAD) for simultaneous determination allopurinol (ALP) and benzbromarone (BNZ). Effective separation was accomplished using Durashell C18 column (4.6 × 250 mm, 5 µm particle size) gradient elution mobile phase composed 0.02 M ammonium acetate (pH 5.0) methanol. Quantification ALP BNZ based on measuring their peak areas at 251 nm. peaks eluted retention times 4.85 10.30 min respectively. proposed procedure carefully validated terms system suitability, linearity, ranges, precision, accuracy, specificity, robustness, detection, quantification limits. linearity range both 5–100 µg/mL correlation coefficients >0.9999. Forced degradation conditions neutral, acidic, alkaline hydrolysis, oxidation, thermal were applied drugs. Good resolution drugs from forced products proved that is stability-indicating. In addition, about 10 pharmacologically or chemically related pharmaceutical compounds different medicinal categories showed high specificity method. successfully tablet dosage forms. Furthermore, greenness assessment comparison previously published methods carried out two GAC metrics, namely, national environmental index (NEMI) Eco-Scale.